A simple and efficient method of genomic DNA extraction and purification from diverse biological samples

Abstract

Genomic DNA (gDNA) is the heritable, genetic information contained in each cell that provides the information needed to develop, maintain, and reproduce an organism. Organized into chromosomes, gDNA is protected by proteins and partitioned into the nucleus in eukaryotic cells. The ability to extract and purify gDNA from samples of interest is an essential first step in the study of many biological processes. There are different methods to extract and purify gDNA such as solution-based, spin column-based, and magnetic beads methods. In this study, gDNA has been extracted and purified from diverse biological samples such as plants, bacteria (gram-negative and gram-positive), human blood, scorpion tissues, and soil sample using a simple and efficient mini-spin column method. The concentration of DNA was determined using a fluorimeter and Nanodrop. The integrity of DNA was determined by gel electrophoresis.