PCR Based Method for Detection of Pathogenic Bacteria in Food

Abstract

PCR-based detection method for three major foodborne pathogens i.e. Salmonella spp., S. aureus, and B. cereus found in milk was established. Salmonella spp., S. aureus, and B. cereus were detected in the singleplex PCR method. Salmonella spp. and S. aureus were also detected in multiplex PCR. Primers of Inv-A, hbl, and ileS genes were employed for the detection of Salmonella spp., B. cereus, and S. aureus respectively. Salmonella was detected at the genus level and the remaining two pathogens were detected at the species level in PCR tests.